Measuring images
11. Pay attention to the displayed results in the preview, and in the
Results
group.
12. If necessary, change the position and size of the intensity range in the
scatterplot.
Then only the colocalization of the pixels that lie within the chosen
intensity range are shown in the preview.
13. Click the
OK
button to finish the measurement of colocalization.
If you haven't changed the default settings for colocalization, a new
image, that contains the colocalization channel, will be created.
At the same time, a workbook that contains the results of the
colocalization measurement, will be displayed. The columns in the
workbook contain the supplement "ROI".
14. If required, use the
File > Save as...
menu command, to save the new image
and the workbook.
15. The multi-channel image will also have been changed when the ROI was
defined. Therefore, if you want to keep the ROI, save it also.
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