Acquiring fluorescence images
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6.3. Defining observation methods for the
fluorescence acquisition
Before you acquire a fluorescence image, you have to define observation
methods for your color channels. As a rule, observation methods that you can
adapt for your microscope configuration, have already been predefined.
The system has already been configured and calibrated. For this purpose, you
have to enter your hardware components in the
Acquire > Devices > Device List
dialog box, and configure them in the
Device Settings
dialog box. To finalize this
action, calibrate your system by using the
Acquire > Calibrations...
command.
Prerequisite
The tables that follow, contain example configurations for both motorized, and
not motorized, microscopes. Only the hardware components that are relevant for
the acquisition of multi-channel fluorescence images are listed.
Example entries
Device List
Non-motorized
microscope
Motorized microscope
Microscope
Frame
<Name of your
microscope>
BX51 BX61
Nosepiece
<Name of your
nosepiece>
Manual Nosepiece
Motorized (UCB)
Mirror Turret
<Name of your mirror
turret>
Manual Mirror
Turret
BX-RFAA
Stage
Z-axis
<Name of your
controller for the
stage's Z-drive>
Not Motorized
Motorized (UCB)
Fluorescence/reflected lightpath
Shutter
<Name of the
reflected light
shutter>
Manual Shutter
Motorized (UCB)
Transmission lightpath
Lamp
<Name of your
transmission lamp>
Not used
UCB Halogen-Lamp
Condenser
<Name of your
condenser>
Manual Condenser
U-UCD8A
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