Acquiring fluorescence images
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14. Click the
Move
button on the
Toolbox
toolbar.
On the image window, the mouse pointer will change its shape.
n Tool
15. Move the whole image with the left mouse button depressed.
16. Click, e.g., the
Selectio
button on the
Toolbox
toolbar, to leave the
mission image and the multi-channel image
lti-
both images.
17. To start with, select the image layer in the
Layers
tool window. To do so,
simply click the layer's name.
18. The layer you have selected will then be shown with a colored background in
the tool window.
19. Then, click the
Set Layer Opacity
move mode.
When you display the trans
simultaneously in the image window, the transmission image will cover the mu
channel image, and for this reason, you can't see the multi-channel image. You
can display both images transparently, and in that way be able to see parts of
button. You will find this button in the
tool window's toolbar.
In the tool window, a slide control will then appear, with which you can
set the degree of transparency.
20. Use the slide control to set the degree of transparency you want. At a value
of 100% the image layer is opaque. At a value of 0% the image layer will be
completely faded out.
21. When you're satisfied with the transparency setting, click once on any place
on the user interface.
22. Use the
File > Save as...
command, to save your new multi-layer image.
Always use the TIF or VSI file format when saving an image.
00067 25072011
Changing the weighting
bet
channel
Saving a multi-layer
image
ween a transmission
image and a multi-
image
