Creating stitched images
Your system now acquires an image at the current position on the
sample. In the image window you now see, on the left (1) the acquired
image, and on the right (2) the live-image is displayed.
Since you haven't moved the sample, the live-image still shows the
current sample position, too, which means that you now see the current
image twice.
The two images overlap. Since the live-image is shown transparent, you
see both images in the overlap area simultaneously.
13. Make a note of a significant structure on the live-image's right border. You
will find the same sample structure in the overlap area. On the illustration, a
significant structure has been indicated by a circle.
14. Now move the stage very slowly to make the structure on the live-image
move to the left. Keep moving the stage until the image structures in the
overlap area lie as exactly over each other as possible. The image structures
need not lie precisely over each other, since your software will match the
individual images with each other.
In the overlap area (3), the same image segments are shown now. This
enables your software to seamlessly combine the two images.
You can reverse the direction in which your stage moves, in the
Device
Settings > Stage
dialog box. Depending on how you can best orient
yourself, the live-image will then move to the left or to the right, when you
move your stage to the right.
15. Check whether both images have been correctly combined. Otherwise you
can undo the last step by using the
Undo last frame
button. You can then
move the stage again, and match the structures better.
During the acquisition, you can change the current stitched image's
zoom factor, e.g., to see certain parts in the overlap area better. You will
find an overview on the possibilities of changing an image's zoom factor
in the online help.
16. Define your way through the sample, with the arrow buttons, and follow that
with the stage.
In this manner, you can display a sample in any form you like in the stitched
image. The illustration shows a stitched image that is made up of 9 individual
images, and the stage path.
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