Creating stitched images
7. Creating stitched images
Acquiring a stitched image without a motorized XY-
stage (Manual MIA)
Example: You want to acquire an image of a large sample area. Use the
Manual
MIA
acquisition process, to acquire several individual images of adjoining
positions on the sample, and to have them combined into a stitched image.
The camera is aligned parallel to the XY-stage. The angle between camera and
stage should be smaller than 1°.
Prerequisite
1. Switch to the "Acquisition" layout. To do this, use, e.g., the
View > Layout >
Acquisition
command.
2. On
the
Microscope Control
toolbar, click the button with the objective that
you want to use for the acquisition of the stitched image.
Selecting the objective
3. Switch to the live mode, and select the optimal settings for your acquisition,
in the
Camera Control
tool window. Pay special attention to setting the
correct exposure time. This exposure time will be used for all of the stitched
image's individual images.
Setting the image
quality
4. Find the position on the sample at which you want to start acquiring the
stitched image.
5. Finish the live mode.
6. Activate
the
Process Manager
tool window.
7. Select
the
Manual Processes
option.
Selecting the
acquisition process
8. Click
the
Manual MIA
button.
The button will appear clicked. You can recognize this status by the
button's colored background.
The
Manual MIA
group will be automatically displayed in the tool window.
Should
the
Instant EFI
acquisition process have been active, it will be
automatically switched off. You can, however, use images with extended
depth of focus for the stitched image. To do this, before you acquire each
of the individual images, click the
Instant EFI
button, located in the
Manual MIA
group.
9. Make quite certain that the
Auto Align
button appears clicked. It should then
look like this:
.
Selecting the
acquisition parameters
Then, your software will search for the same image structures in
neighboring individual images. The stitched image will be put together in
such a way that image areas that are the same will be superimposed.
10. Click the
Start
button.
Acquiring a stitched
image
Your software switches into the live mode.
11. Bring the sample into focus.
12. Click on one of the arrow buttons to set the side of the current image at
which the next image is to be arranged. For example, click this button
if
the next image is to be laid to the right of the current image.
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